Free Mammary Stem Cells Methods and Protocols (Methods in Molecular Biology)
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Book Details :
Published on: 2015-06-04
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Original language: English
The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer. The Epithelial-Mesenchymal Transition Generates Cells with ... Figure 1. The Epithelial-Mesenchymal Transition (EMT) Generates Cells with Properties of Stem Cells (A) Phase-contrast images of HMLE cells expressing Snail Twist ... Generation of cerebral organoids from human pluripotent ... Generation of cerebral organoids from human pluripotent stem cells. Madeline A Lancaster 1 Juergen A Knoblich 1 Affiliations; Contributions; Corresponding authors Organoid - Wikipedia An organoid is a miniaturized and simplified version of an organ produced in vitro in three dimensions that shows realistic micro-anatomy. They are derived from one ... Protease Inhibitor Cocktails References Sigma-Aldrich Broad spectrum protease inhibitors and mixtures or Cocktails have been developed to help protect the integrity of proteins during the protein extraction and ... The Hippo Transducer TAZ Confers Cancer Stem Cell-Related ... Summary. Cancer stem cells (CSCs) are proposed to drive tumor initiation and progression. Yet our understanding of the cellular and molecular mechanisms that ... Induced stem cells - Wikipedia Induced stem cells (iSC) are stem cells derived from somatic reproductive pluripotent or other cell types by deliberate epigenetic reprogramming. Nature Reviews Molecular Cell Biology Growth arrest. The hallmark of cellular senescence is an inability to progress through the cell cycle. Senescent cells arrest growth usually with a DNA content that ... RefWorks: Products Output Style List. RefWorks offers hundreds of output styles including APA MLA Chicago Vancouver and Turabian and we are continually adding more. Apoptosis in differentiating C2C12 muscle cells Hematopoietic stem cells (HSCs) are maintained through the regulation of symmetric and asymmetric cell division. myeloid reconstitution. Hence Msi2 can be an ... Primary Cells World's Largest Provider of Primary Cells ... ScienCell is proud to provide researchers with over 140 types of reliable high quality primary cells from 24 normal human and animal systems including nervous ...
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